Transport of D-xylose in Lactobacillus pentosus, Lactobacillus casei and Lactobacillus plantarum: Evidence for a Mechanism of Facilitated Diffusion via the Phosphoenolpyruvate:Mannose Phosphotransferase System
ثبت نشده
چکیده
Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.
منابع مشابه
Transport of D-xylose in Lactobacillus pentosus, Lactobacillus casei, and Lactobacillus plantarum: evidence for a mechanism of facilitated diffusion via the phosphoenolpyruvate:mannose phosphotransferase system.
We have identified and characterized the D-xylose transport system of Lactobacillus pentosus. Uptake of D-xylose was not driven by the proton motive force generated by malolactic fermentation and required D-xylose metabolism. The kinetics of D-xylose transport were indicative of a low-affinity facilitated-diffusion system with an apparent K(m) of 8.5 mM and a V(max) of 23 nmol min(-1) mg of dry...
متن کاملFunctional expression in Lactobacillus plantarum of xylP encoding the isoprimeverose transporter of Lactobacillus pentosus.
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown...
متن کاملContribution of the phosphoenolpyruvate:mannose phosphotransferase system to carbon catabolite repression in Lactobacillus pentosus.
The role of the Lactobacillus pentosus phosphoenolpyruvate:mannose phosphotransferase system (mannose PTS) in sugar transport and control of sugar utilization was investigated. Growth experiments and measurements of PEP-dependent phosphorylation of sugars, of sugar transport and of catabolic enzyme activity were performed, to compare a wild-type strain with an EIIB(Man) mutant, LPE6, and a ccpA...
متن کاملAntibacterial Activity of Lactobacillus plantarum and Lactobacillus casei on Burkholderia cepacia Isolated from Imam Ali Hospital
Background: Nosocomial infections are the causes of the most common of morbidity and mortality, prolonged hospital stay, and increased hospital costs worldwide. Today probiotic bacteria, as antibacterial agents, attracted the attention of clinicians. The current study aimed at determining the antibacterial activity of Lactobacillus plantarum and L. casei species against Burkholderia cepacia iso...
متن کاملGlucose transport by the phosphoenolpyruvate:mannose phosphotransferase system in Lactobacillus casei ATCC 393 and its role in carbon catabolite repression.
A 2-deoxy-D-glucose-resistant mutant of a pLZ15-cured derivative of Lactobacillus casei ATCC 393 was isolated on agar medium containing 10 mM 2-deoxy-D-glucose and 5 g lactose I-1. The mutant was impaired in the main glucose transport mechanism, a PTSman-type system. Additionally a proton-motive-force-dependent glucose permease was detected. The growth response and the sugar consumption rates o...
متن کامل